mCherry Antibody

SKU: orb613681

Description

Our Anti-mCherry primary antibody from Biorbyt is mouse monoclonal. It detects mCherry and is Protein G purified.

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 0.388888889, ICC: 0.388888889

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 1C51
• Immunogen: Recombinant full length protein
• Target: mCherry
• Molecular Weight: 28
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 50% glycerol and 5 mM NaN3
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein

Immunostaining of HEK293 cells transfected with mCherry(red) and stained with anti-mCherry antibody (Cat. orb613681, green, 1:500) subsequently appear yellow. The nuclei stained blue (DAPI) distinguishes untransfected cells.

Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).

Quick Database Links

Gene Symbol

mCherry