Laminin alpha 5 Antibody

• Catalog Number: orb214177
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LAMA5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Laminin alpha 5. The exact sequence is proprietary.
• UniProt ID: Q61001, O15230
• Tested applications: IHC, IP, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIAA0533; KIAA1907; Laminin subunit alpha-5; Lamin
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 16776, 3911
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Laminin alpha 5 expression in JAR (A), A431 (B), RAW264.7 (C) whole cell lysates. (Predicted band size: 399 kD; Observed band size: 400 kD)

Immunohistochemical analysis of Laminin alpha 5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.