Laminin α-5 rabbit pAb Antibody

SKU: orb768952

Description

Rabbit polyclonal antibody to Laminin alpha-5

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human LAMA5. AA range:2381-2430
• Molecular Weight: 400
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-LAMA5 antibody, anti-KIAA0533 antibody, anti-KIAA1907 antibody, anti-Laminin subunit alpha-5 antibody, anti-Laminin-10 subunit alpha antibody, anti-Laminin-11 subunit alpha antibody, anti-Laminin-15 subunit alpha antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western Blot analysis of RAW HepG2 A549 cells using Laminin α-5 Polyclonal Antibody.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LAMA5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night.