Laminin 5 Rabbit Polyclonal Antibody

SKU: orb100754

Description

Laminin 5 Rabbit Polyclonal Antibody

Research Area

Basal Lamina, Cancer Research, Cell Biology, Intermediate Filaments, Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LAMA3 (2701-2900/3333aa)
• Target: LAMA3
• Molecular Weight: 367 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BM600; E170; JEB2A; JEB2B; JEB2C; LAMNA; LOCS; Lama3B; [a]3B; LAMA3_HUMAN; LAMA3; Epiligrin 170 kDa subunit (E170); Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha; LAMA3_MOUSE;

Tissue/cell: mouse stomach wall, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated (orb100754) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

LAMA3

UniProt

Q16787

Tetsuto Yamaura 1 2, Mao Watanabe 1 3, Yusuke Minato 1, Rika Sakuma 1, Seishi Maeda 1, Toshiya Tachibana 2, Hideshi Yagi 1 Laminin 332 regulates glial fibrillary acidic protein expression and astrocyte maturation during spinal cord development J Anat, (2025)

PubMed: 40930803

Applications

IF

Reactivity

Mouse