Ki67 Antibody

• Catalog Number: orb705451
• Category: Antibodies
• Description: The Ki67 Antibody is suitable for IF, IHC. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within human Ki67. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Ki67. The exact sequence is proprietary.
• UniProt ID: P46013
• Tested applications: IF, IHC
• Dilution range: IHC-P: 1:100-1:300, IF: 1:100-1:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Antigen KI-67
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 4288
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of Ki67 staining in human classical hodgkin's lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Ki67 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.