Ki-67 (Proliferating Cell Marker) Antibody

• Catalog Number: orb751175
• Category: Antibodies
• Description: Mouse monoclonal antibody against MKI67 conjugated to Biotin
• Target: MKI67
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Immunogen: Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary)
• UniProt ID: P46013
• MW: 345kDa and 395kDa
• Tested applications: FC, IF, IHC
• Dilution range: Flow Cytometry (5ul per test per million cells); Immunofluorescence (2-4ug/ml at RT);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: Any actively proliferating cells. Human skin, tonsil or lymph node.
• Clone Number: MKI67/2465
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-KI-67 antibody, anti-Ki67 antibody, anti-KI-6
• Note: For research use only
• Entrez: 4288