MKI67 Antibody (Biotin)

SKU: orb751175

Description

Mouse monoclonal antibody against MKI67 conjugated to Biotin

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution Range: Flow Cytometry (5ul per test per million cells); Immunofluorescence (2-4ug/ml at RT);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Any actively proliferating cells. Human skin, tonsil or lymph node.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: MKI67/2465
• Immunogen: Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary)
• Target: MKI67
• Source/Expression System: Bioreactor
• Molecular Weight: 345kDa and 395kDa
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-KI-67 antibody, anti-Ki67 antibody, anti-KI-67 Antigen (KIA) antibody, anti-MKI67 antibody, anti-Proliferation related Ki-67 antigen antibody

Quick Database Links

Gene Symbol

MKI67

UniProt

P46013

NCBI Gene ID

4288