Ki-67 Antibody

• Catalog Number: orb257046
• Category: Antibodies
• Description: Mouse monoclonal antibody to Ki-67
• Target: Ki-67
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Bovine, Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Nuclei of the Hodgkin lymphoma cell line L428
• UniProt ID: P46013
• Tested applications: FC, ICC, IHC, WB
• Application notes: Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: Ki-67
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIA, MIB, Marker of proliferation Ki67, MKI67
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 4288
• Expiration Date: 12 months from date of receipt.

Separation of human Ki-67 positive cells (red-filled) from Ki-67 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.

Flow cytometry intracellular staining pattern of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.

Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).