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Anti-Ki-67 Purified
Catalog Number: orb257046
| Catalog Number | orb257046 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to Ki-67 |
| Clonality | Monoclonal |
| Clone Number | Ki-67 |
| Tested applications | FC, ICC, IHC, WB |
| Reactivity | Bovine, Human |
| Isotype | Mouse IgG1 |
| Immunogen | Nuclei of the Hodgkin lymphoma cell line L428 |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | Ki-67 |
| Entrez | 4288 |
| UniProt ID | P46013 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti Ki-67 antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-12 µg/ml. Intracellular staining.Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Separation of human Ki-67 positive cells (red-filled) from Ki-67 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.
Flow cytometry intracellular staining pattern of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.
Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).
