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Anti-IGF2BP1 Antibody

Catalog Number: orb378121

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb378121
CategoryAntibodies
DescriptionRabbit polyclonal antibody to IGF2BP1.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide of human IGF2BP1
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000, IHC-P: 1:50-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetIGF2BP1
Entrez303477, 10642, 140486
UniProt IDQ9NZI8, O88477, Q8CGX0
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesCRDBP; VICKZ1; ZBP1; Insulin-like growth factor 2
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-IGF2BP1 Antibody

Western blot analysis of IGF2BP1 expression in HL60 (A), K562 (B), NIH3T3 (C), mouse liver (D) whole cell lysates. (Predicted band size: 48; 63 kD; Observed band size: 71 kD)

Anti-IGF2BP1 Antibody

Immunohistochemical analysis of IGF2BP1 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-IGF2BP1 Antibody

Immunofluorescent analysis of IGF2BP1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    50 μg, 100 μg
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    ELISA

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