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Catalog Number | orb44227 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to PAG |
Clonality | Monoclonal |
Clone Number | MEM-255 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG). |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | PAG1 |
Entrez | 55824 |
UniProt ID | Q9NWQ8 |
RRID | AB_10994343 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | PAG antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 2 μg/ml. Intracellular staining.Immunohistochemistry (paraffin sections): Positive tissue: tonsil, spleen. Western blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which, however, migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255). Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.