PAG1 Rabbit Polyclonal Antibody

• Catalog Number: orb158091
• Category: Antibodies
• Description: PAG1 Rabbit Polyclonal Antibody
• Target: PAG1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Canine, Equine, Human, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human PAG1 (331-432/432aa)
• UniProt ID: Q9NWQ8
• MW: 47 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CBP; Csk Binding Protein; Csk-binding protein; FLJ
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phosphoprotein associated with glycosphingolipid enriched microdomains 1, PAG1) Polyclonal Antibody, Unconjugated (orb158091) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Spleen tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-PAG1 (orb158091) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa.