Anti-Hu IgE Purified

• Catalog Number: orb44850
• Category: Antibodies
• Description: Mouse Monoclonal to Human IgE.
• Target: IgE
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Immunogen: Purified human IgE.
• RRID: AB_10997022
• Tested applications: ELISA, FC
• Application notes: Flow cytrometry: Recommended dilution: 1-4 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: 4G7.325
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-IgE antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Separation of human IgE positive basophil granulocytes (red-filled) from neutrofil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgE (4G7.325) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (4G7.325) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).