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Catalog Number | orb182383 |
---|---|
Category | Antibodies |
Description | Anti-CD23/FCER2 Antibody |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Purification | Immunogen affinity purified. |
Immunogen | E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23. |
UniProt ID | P20693 |
MW | 49 kDa |
Tested applications | ELISA, FC, ICC, IF, IHC, IHC-Fr, WB |
Application notes | Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry, 0.5-1μg/ml, Human Immunofluorescence, 2μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse ELISA (Cap), 1-5μg/ml, Mouse. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Cross Reactivity | No cross-reactivity with other proteins |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Low affinity immunoglobulin epsilon Fc receptor; F Read more... |
Note | For research use only |
Flow Cytometry analysis of mouse PBMC cells using anti-CD23 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of mouse BMC cells using anti-CD23 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CD23 using anti-CD23 antibody.Lane 1:Mouse Liver Tissue.
WB analysis of CD23 using anti-CD23 antibody.Lane 1:rat spleen tissue;2:rat thymus tissue.
IF analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of mouse lymphaden tissues.
IF analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of rat lymphaden tissues.
Sandwich ELISA - Recombinant mouse CD23/FCER2 protein standard curve.Use in combination with reagents from Mouse CD23/FCER2 ELISA Kit EZ-Set (DIY Antibody Pairs).
IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Human Tonsil Tissue.
IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Mouse Spleen Tissue.
IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Rat Spleen Tissue.
IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in frozen section of mouse spleen tissues.
FC, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
iFluor647 |
ELISA, FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE |
ELISA, FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC |