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Product Overview
Product Name CD20 antibody
Catalog Number orb10307
Species/HostRabbit
ReactivityHuman, Mouse, Rat
ConjugationUnconjugated
Tested applicationsIHC-P, P-ELISA, WB
Immunogen KLH conjugated synthetic peptide derived from human CD20. Please contact us for the exact immunogen sequence. The peptide is available as orb374711.
Target CD20
Alternative Names
Product Properties
Form/Appearance 10 mM PBS, 0.02% sodium azide
Concentration - 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Polyclonal antibodies are purified by peptide affinity chromatography
MW 33 kDa
Uniprot ID P11836 (Human); P19437 (Mouse)
NCBI NP_068769.21; NM_021950.31
Entrez 931
Product Description

CD20 antigen is a type of tumor marker. Also called CD20. There's a protein found on B cells (a type of white blood cell). It may be found in higher than normal amounts in patients with certain types of B-cell lymphomas and leukemias. Measuring the amount of CD20 antigen on blood cells may help to diagnose cancer or plan cancer treatment.The protein has no known natural ligand[8] and its function is to enable optimal B-cell immune response, specifically against T-independent antigens.It is suspected that it acts as a calcium channel in the cell membrane.It has been shown that CD20 also plays a role in the microenvironmental interactions of B cells.

Application Notes
Dilution Range WB: 1:100-500, IHC-P: 1:100-800 (based on 0.5 mg/ml)
Validation Images
WB analysis of human thyroid tumor (lane 1), human breast cancer (lane 2), human thyroid cancer (lane 3) using CD20 antibody (1 ug/ml)
WB analysis of human thyroid tumor (lane 1), human breast cancer (lane 2), human thyroid cancer (lane 3) using CD20 antibody (1 ug/ml)
Immunohistochemical staining of rat lymph node tissue using anti-CD20 (dilution of primary antibody - 2.5 ug/ml)
Immunohistochemical staining of rat lymph node tissue using anti-CD20 (dilution of primary antibody - 2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat lymph node tissue using anti-CD20 (primary antibody at 2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat lymph node tissue using anti-CD20 (primary antibody at 2.5 ug/ml)
Immunohistochemical staining of rat lymph node tissue using anti-CD20 (dilution of primary antibody - 2.5 ug/ml)
Immunohistochemical staining of rat lymph node tissue using anti-CD20 (dilution of primary antibody - 2.5 ug/ml)
IHC-P image of rat pancreas tissue using anti-CD20 (dilution of primary antibody at 2.5 ug/ml)
IHC-P image of rat pancreas tissue using anti-CD20 (dilution of primary antibody at 2.5 ug/ml)
IHC-P staining of rat spleen tissue using CD20 antibody (dilution at 2.5 ug/ml)
IHC-P staining of rat spleen tissue using CD20 antibody (dilution at 2.5 ug/ml)
IHC-P image of rat spleen tissue using CD20 antibody (dilution of primary antibody at 2.5 ug/ml)
IHC-P image of rat spleen tissue using CD20 antibody (dilution of primary antibody at 2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat pancreas tissue using anti-CD20 (primary antibody at 2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat pancreas tissue using anti-CD20 (primary antibody at 2.5 ug/ml)
WB analysis of mouse lymph node (lane 1); mouse pacreas (lane 2); mouse spleen (lane 3) using CD20 antibody (1 ug/ml)
WB analysis of mouse lymph node (lane 1); mouse pacreas (lane 2); mouse spleen (lane 3) using CD20 antibody (1 ug/ml)
IHC-P image of human endometrial cancer tissue using CD20 antibody (dilution of primary antibody at 2.5 ug/ml)
IHC-P image of human endometrial cancer tissue using CD20 antibody (dilution of primary antibody at 2.5 ug/ml)
Western blot analysis of human thyroid tumor (lane 1), human breast cancer (lane 2), human thyroid cancer (lane 3) using CD20 antibody (1 ug/ml)
Western blot analysis of human thyroid tumor (lane 1), human breast cancer (lane 2), human thyroid cancer (lane 3) using CD20 antibody (1 ug/ml)
References
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