CD82 Antibody

SKU: orb154380

Description

Mouse monoclonal antibody to CD82 which is a member of the tetraspanin (TM4SF) family. It localized in Lymphoid specific. It associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. CD82 has since been found to act as a metastasis suppressor in a variety of cancers, and its downregulation is associated with poor prognosis in research studies.

Images & Validation

• Tested Applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: C33
• Immunogen: C91/PL (human HTLV-1+ T cell line)
• Target: CD82
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27

Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 µg/ml) GAM APC.

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).

Quick Database Links

Gene Symbol

CD82

UniProt

P27701

NCBI Gene ID

3732