Anti-Hu CD82 Purified

• Catalog Number: orb154380
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD82 which is a member of the tetraspanin (TM4SF) family. It localized in Lymphoid specific. It associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. CD82 has since been found to act as a metastasis suppressor in a variety of cancers, and its downregulation is associated with poor prognosis in research studies.
• Clonality: Monoclonal
• Clone Number: C33
• Tested applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: C91/PL (human HTLV-1+ T cell line)
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD82
• Entrez: 3732
• UniProt ID: P27701
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-R2 antibody, anti-4F9 antibody, anti-C33 anti
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 µg/ml) GAM APC.

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).