CD81 Antibody

• Catalog Number: orb699624
• Category: Antibodies
• Description: Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rC81/3442
• Tested applications: FACS, Functional Studies, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human CD81 protein was used as the immunogen for the recombinant CD81 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Functional Studies: order Ab format without BSA/sodium azide,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 1-2ug/ml for 2 hours at RT,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD81 antibody is available for research use only.
• UniProt ID: P60033
• Storage: Store the recombinant CD811 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry staining of human MCF7 cells with recombinant CD81 antibody; Gray=unstained cells, Orange= recombinant CD81 antibody.

Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone rC81/3442). Gray=unstained cells, Orange = recombinant CD81 antibody.

IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.