CD81 Antibody

SKU: orb699624

Description

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Images & Validation

• Tested Applications: FACS, Functional Studies, IF, IHC-P, WB
• Dilution range: Functional Studies: order Ab format without BSA/sodium azide,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 1-2ug/ml for 2 hours at RT,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rC81/3442
• Immunogen: Recombinant full-length human CD81 protein was used as the immunogen for the recombinant CD81 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Flow cytometry staining of human MCF7 cells with recombinant CD81 antibody; Gray=unstained cells, Orange= recombinant CD81 antibody.

Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone rC81/3442). Gray=unstained cells, Orange = recombinant CD81 antibody.

IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P60033