CD82 (ST6) Antibody (C-term)

• Catalog Number: orb1930154
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB02073-02074
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 29626 Da
• Target: This CD82 (ST6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the C-terminal region of human CD82 (ST6).
• UniProt ID: P27701
• NCBI: NP_002222.1, NP_001020015.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CD82 antigen, C33 antigen, IA4, Inducible membrane
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HepG2 cells using CD82 (ST6) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lymph with CD82 (ST6) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of ST6 (arrow) using ST6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD82 gene (Lane 2).

Anti-ST6 Antibody (C253) at 1:1000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ST6 Antibody (C253) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.