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Anti-Hu CD45 Purified
Catalog Number: orb44725
| Catalog Number | orb44725 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to CD45 |
| Target | CD45 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Conjugation | Unconjugated |
| Reactivity | Human, Primate |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified by protein-G affinity chromatography. |
| Immunogen | human peripheral blood leukocytes |
| UniProt ID | P08575 |
| RRID | AB_10999116 |
| Tested applications | FC, ICC, IHC-Fr, IHC-P, IP, WB |
| Dilution range | Western blotting: Recommended dilution: 1 μg/ml; non-reducing conditions, band around 200-250 kDa; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel. Flow cytometry: Recommended dilution: 1-4 µg/ml |
| Application notes | Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).Flow cytometry: Recommended dilution: 1-4 µg/ml |
| Antibody Type | Primary Antibody |
| Clone Number | HI30 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti CD 45 antibody, anti CD45 antibody, anti CD45 Read more... |
| Note | For research use only |
| Entrez | 5788 |
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (HI30) purified, GAM-APC.
Anti-Hu CD45 Purified (clone HI30) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody HI30 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.
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