You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1294367 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CD45 antibody (Extracellular domain) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human CD45 |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:400-1:800 |
Conjugation | Unconjugated |
Target | CD45 (Extracellular) |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4°C.
Product: CD45 antibody; Catalog: orb1294367; Cell: THP-1.
Immunohistochemical analysis of paraffin embedded Human Colorecta cancer tissue labeling CD45 with orb1294367 at 1/400.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294367 anti-CD45 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294367 at 1/400.
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294367 at 1/400.
Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294367 at 1/400.
FACS, IF, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |