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CD45 (Extracellular)

Catalog Number: orb1294367

DispatchUsually dispatched within 5-10 working days
$ 260.00
Catalog Numberorb1294367
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CD45 antibody (Extracellular domain)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC, WB
ReactivityHuman, Mouse
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / Extracellular domain corresponding to Human CD45
Dilution rangeWestern Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:400-1:800
ConjugationUnconjugated
TargetCD45 (Extracellular)
StorageStore at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
NoteFor research use only
Expiration Date12 months from date of receipt.
CD45 (Extracellular)

All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4°C.

CD45 (Extracellular)

Product: CD45 antibody; Catalog: orb1294367; Cell: THP-1.

CD45 (Extracellular)

Immunohistochemical analysis of paraffin embedded Human Colorecta cancer tissue labeling CD45 with orb1294367 at 1/400.

CD45 (Extracellular)

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294367 anti-CD45 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

CD45 (Extracellular)

All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4°C.

CD45 (Extracellular)

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294367 at 1/400.

CD45 (Extracellular)

Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294367 at 1/400.

CD45 (Extracellular)

Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294367 at 1/400.

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