CD45 (Extracellular) Rabbit Polyclonal Antibody

SKU: orb1294367

Description

Rabbit polyclonal antibody to CD45 antibody (Extracellular domain)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1:400-1:800
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD45
• Target: CD45 (Extracellular)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4°C.

All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294367 anti-CD45 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294367 at 1/400.

Immunohistochemical analysis of paraffin embedded Human Colorecta cancer tissue labeling CD45 with orb1294367 at 1/400.

Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294367 at 1/400.

Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294367 at 1/400.

CD45 antibody, orb1294367, Cell: THP-1.

Quick Database Links

Gene Symbol

CD45 (Extracellular)