CD45 (Intracellular)

• Catalog Number: orb1294366
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD45 antibody (Intracellular domain)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human CD45
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1: 200-1:400 Flow Cytometry 1:50-1:100
• Conjugation: Unconjugated
• Target: CD45 (Intracellular)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294366 overnight at 4°C.

Product: CD45 antibody; Catalog: orb1294366; Cell: THP-1.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling CD45 with orb1294366 at 1/300.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294366 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294366 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294366 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294366 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294366 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Lymph node carcinoma labeling CD45 with orb1294366 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Squamous cell carcinoma labeling CD45 with orb1294366 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling CD45 with orb1294366 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Ovary cancer tissue labeling CD45 with orb1294366 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Gastric cancer tissue labeling CD45 with orb1294366 at 1/300.