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CD45 (Intracellular)

Catalog Number: orb1294366

DispatchUsually dispatched within 5-10 working days
$ 260.00
Catalog Numberorb1294366
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CD45 antibody (Intracellular domain)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC, WB
ReactivityHuman
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / Intracellular domain corresponding to Human CD45
Dilution rangeWestern Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1: 200-1:400 Flow Cytometry 1:50-1:100
ConjugationUnconjugated
TargetCD45 (Intracellular)
StorageStore at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
NoteFor research use only
Expiration Date12 months from date of receipt.
CD45 (Intracellular)

All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294366 overnight at 4°C.

CD45 (Intracellular)

Product: CD45 antibody; Catalog: orb1294366; Cell: THP-1.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294366 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

CD45 (Intracellular)

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294366 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Lymph node carcinoma labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Squamous cell carcinoma labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Ovary cancer tissue labeling CD45 with orb1294366 at 1/300.

CD45 (Intracellular)

Immunohistochemical analysis of paraffin embedded Human Gastric cancer tissue labeling CD45 with orb1294366 at 1/300.

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