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Anti-Hu CD45 Purified

Catalog Number: orb44266

DispatchUsually dispatched within 5-10 working days.
$ 260.00
Catalog Numberorb44266
CategoryAntibodies
DescriptionMouse monoclonal antibody to CD45
ClonalityMonoclonal
Clone NumberMEM-28
Tested applicationsFC, ICC, IHC-P, IP, WB
ReactivityHuman
IsotypeMouse IgG1
ImmunogenHuman thymocytes and T lymphocytes.
Antibody TypePrimary Antibody
Concentration1 mg/ml
Dilution rangeImmunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used. Western blotting: Recommended dilution: 1 μg/ml; non-reducing conditions, band around 200-250 kDa; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel. Flow cytometry: Recommended dilution: 1 μg/ml.
PurityPurified by protein-A affinity chromatography.
ConjugationUnconjugated
TargetCD45
Entrez5788
UniProt IDP08575
RRIDAB_10991731
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alternative namesanti CD 45 antibody, anti CD45 antibody, anti CD45
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NoteFor research use only
Application notesImmunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used. Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa). Flow cytometry: Recommended dilution: 1 μg/ml.
Expiration Date12 months from date of receipt.
Anti-Hu CD45 Purified

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (MEM-28) purified, GAM-APC.

Anti-Hu CD45 Purified

Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28, green). DNA visualized by DAPI (blue)

Anti-Hu CD45 Purified

Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-CD45 (MEM-28), 10 µg/ml.

Anti-Hu CD45 Purified

Anti-Hu CD45 Purified (clone MEM-28) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-28 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.

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    Monoclonal

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    0.1 mg
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    FC,  ICC,  IHC-Fr,  IHC-P,  IP,  WB

    Human, Primate

    Monoclonal

    Unconjugated

    0.1 mg