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Anti-Hu CD45 Purified

Catalog Number: orb233593

DispatchUsually dispatched within 5-10 working days
$ 260.00
Catalog Numberorb233593
CategoryAntibodies
DescriptionMouse monoclonal antibody to CD45
ClonalityMonoclonal
Clone Number2D1
Tested applicationsFC, WB
ReactivityHuman
IsotypeMouse IgG1 kappa
ImmunogenHuman peripheral blood mononuclear cells
Antibody TypePrimary Antibody
Concentration1 mg/ml
Dilution rangeFlow cytometry: Recommended dilution: 1-4 μg/ml.
PurityPurified by protein-A affinity chromatography.
ConjugationUnconjugated
TargetCD45
Entrez5788
UniProt IDP08575
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alternative namesanti CD 45 antibody, anti CD45 antibody, anti CD45
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NoteFor research use only
Application notesFlow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).
Expiration Date12 months from date of receipt.
Anti-Hu CD45 Purified

Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD45 (2D1) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Anti-Hu CD45 Purified

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Anti-Hu CD45 Purified

Anti-Hu CD45 Purified (clone 2D1) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody 2D1 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.

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    FC,  ICC,  IHC-P,  IP,  WB

    Human

    Monoclonal

    Unconjugated

    0.1 mg
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    FC,  ICC,  IHC-Fr,  IHC-P,  IP,  WB

    Human, Primate

    Monoclonal

    Unconjugated

    0.1 mg