Anti-Hu CD45 Purified

• Catalog Number: orb233593
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD45
• Target: CD45
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified by protein-A affinity chromatography.
• Immunogen: Human peripheral blood mononuclear cells
• UniProt ID: P08575
• Tested applications: FC, WB
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).
• Antibody Type: Primary Antibody
• Clone Number: 2D1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CD 45 antibody, anti CD45 antibody, anti CD45
• Note: For research use only
• Entrez: 5788

Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD45 (2D1) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Anti-Hu CD45 Purified (clone 2D1) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody 2D1 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.