CD35 Antibody

SKU: orb154405

Description

Mouse monoclonal antibody to CD35

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IHC-P, IP
• Reactivity: Human, Primate
• Application Notes:
  Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval. Immunohistochemistry (frozen sections): Acetone fixation. Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: E11
• Immunogen: Acute monocytic leukemia cells and normal blood monocytes
• Target: CD35
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CR1, KN, C3BR, C4BR

Separation of human CD35 positive lymphocytes (red-filled) from CD35 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD35 (E11) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD35 (E11) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Quick Database Links

Gene Symbol

CD35

UniProt

P17927

NCBI Gene ID

1378