Anti-Hu CD3 Purified

• Catalog Number: orb44560
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD3
• Clonality: Monoclonal
• Clone Number: UCHT1
• Tested applications: FC, ICC, IHC-Fr, IP
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: human thymocytes followed by Sezary T cells
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD3
• Entrez: 916
• UniProt ID: P07766
• RRID: AB_10995917
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 3 antibody, anti Cd247 antibody, anti CD24
• Note: For research use only
• Application notes: Immunocytochemistry and immunohistochemistry (frozen sections): The epitope for UCHT1 is resistant to fixation. Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml.
• Expiration Date: 12 months from date of receipt.

Mass cytometry (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD3 (UCHT1) Yb174. Gated on singlets.

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Immunocytochemistry staining of human CD3 epsilon and CD3 gamma transfected COS cells using mouse monoclonal antibodies anti-CD3, clones UCHT1 (left) and TB3 (right) indicated by Alexa Fluor 555 signal (red); DNA stained by DAPI (blue).