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Catalog Number | orb44559 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD3 |
Clonality | Monoclonal |
Clone Number | UCHT1 |
Tested applications | FA, FC, IHC-Fr, IP |
Reactivity | Human, Primate |
Isotype | Mouse IgG1 |
Immunogen | human thymocytes followed by Sezary T cells |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD3 |
Entrez | 916 |
UniProt ID | P07766 |
RRID | AB_10999309 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
Alternative names | anti CD 3 antibody, anti Cd247 antibody, anti CD24 Read more... |
Note | For research use only |
Application notes | Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications |
Expiration Date | 12 months from date of receipt. |
Mass cytometry (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD3 (UCHT1) Yb174. Gated on singlets.
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) purified antibody (low endotoxin, concentration in sample 2 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) purified antibody (low endotoxin, concentration in sample 2 μg/ml) GAM APC.