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Anti-Hu CD25 Purified
Catalog Number: orb44155
| Catalog Number | orb44155 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to CD25 |
| Clonality | Monoclonal |
| Clone Number | MEM-181 |
| Tested applications | FC |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Dilution range | Flow cytometry: Recommended dilution: 2 μg/ml. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD25 |
| Entrez | 3559 |
| UniProt ID | P01589 |
| RRID | AB_10992065 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti CD25 antibody, anti CD 25 antibody, anti IL 2 Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 2 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Separation of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD25 (MEM-181) purified antibody (concentration in sample 2 μg/ml, GAM APC, red-filled) from human PHA stimulated peripheral blood mononuclear cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD25 (MEM-181) purified antibody (concentration in sample 2 μg/ml) GAM APC.
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