CD25 antibody

SKU: orb389314

Description

CD25 antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-P, WB
• Dilution range: WB: 1:100-500, IHC-P: 1:100-500, IF/ICC: 1:100-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD25. Please contact us for the exact immunogen sequence. The peptide is available as orb13468.
• Target: CD25
• Molecular Weight: 31 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Immunohistochemical staining of paraffin embedded rat spleen tissue using CD25 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat lymph node tissue using CD25 antibody (2.5 ug/ml)

IF image of rat lymph node tissue using anti-CD25 (2.5 ug/ml)

Western blot analysis of rat lipocyte (lane 1), rat spleen (lane 2), rat small intestines (lane 3), rat lymph node (lane 4), hela cells (lane 5), human lung cancer (lane 6) using CD25 antibody (1 ug/ml)

IHC-P image of human ovarian cancer tissue using anti-CD25 (2.5 ug/ml)

IF analysis of rat lymph node tissue using anti-CD25 (2.5 ug/ml)

Immunohistochemical staining of human lung cancer tissue using CD25 antibody (2.5 ug/ml)

Quick Database Links

Gene Symbol

CD25

UniProt

P25094, P01589, P01590

RefSeq:000408