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Anti-Hu CD25 Purified
Catalog Number: orb44071
| Catalog Number | orb44071 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to CD25 |
| Clonality | Monoclonal |
| Clone Number | MEM-140 |
| Tested applications | FC, IP |
| Reactivity | Human |
| Isotype | Mouse IgM |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Conjugation | Unconjugated |
| Target | CD25 |
| Entrez | 3559 |
| UniProt ID | P01589 |
| RRID | AB_10991410 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Alternative names | anti CD25 antibody, anti CD 25 antibody, anti IL 2 Read more... |
| Note | For research use only |
| Application notes | Immunoprecipitation: Excellent for immunoisolation of CD25+ cells. Flow cytometry: Recommended dilution: 1 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononclear cells stained using anti-human CD25 (MEM-140) purified antibody (concentration in sample 0.6 µg/ml, GAM APC, red) and human PHA stimulated peripheral blood mononclear cells unstained by primary antibody (GAM APC, blue).
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