CD25 Antibody

SKU: orb44071

Description

Mouse monoclonal antibody to CD25

Images & Validation

• Tested Applications: FC, IP
• Reactivity: Human
• Application Notes:
  Immunoprecipitation: Excellent for immunoisolation of CD25+ cells. Flow cytometry: Recommended dilution: 1 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: MEM-140
• Immunogen: PHA-activated peripheral blood leucocytes
• Target: CD25
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

IL-2R α chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononclear cells stained using anti-human CD25 (MEM-140) purified antibody (concentration in sample 0.6 µg/ml, GAM APC, red) and human PHA stimulated peripheral blood mononclear cells unstained by primary antibody (GAM APC, blue).

Quick Database Links

Gene Symbol

CD25

UniProt

P01589

NCBI Gene ID

3559