CD133 Antibody

SKU: orb545653

Description

Anti-Hu CD133 Purified

Images & Validation

• Tested Applications: FC, IHC, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions preferred.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: W6B3C1
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Target: CD133
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prominin 1, PROM1, AC133, PROML1, STGD4

Flow cytometry analysis (surface staining) of human peripheral whole blood showing CD133 positive stem cells (blue) and lymphocytes (red) stained using anti-CD133 (W6B3C1) purified, GAM-FITC.

Western blotting analysis of human CD133 using mouse monoclonal antibody W6B3C1 on lysates of HT29 cells and Jurkat cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD133 was detected around 125 kDa.

Quick Database Links

Gene Symbol

CD133

UniProt

O43490

NCBI Gene ID

8842