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Anti-Hu CD105 Purified
Catalog Number: orb44185
| Catalog Number | orb44185 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to CD105. |
| Clonality | Monoclonal |
| Clone Number | MEM-229 |
| Tested applications | FC, ICC, IHC-Fr, WB |
| Reactivity | Human, Porcine |
| Isotype | Mouse IgG2a |
| Immunogen | Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD105 |
| Entrez | 2022 |
| UniProt ID | P17813 |
| RRID | AB_10993792 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-CD105 antibody, anti-Endoglin antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 2-6 μg/ml; positive control: Kg1 human acute myelogenous leukemia cell line. Immunohistochemistry (frozen sections): Recommended dilution: 1:200, acetone fixation. Western blotting: Non-reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
Separation of HUVEC cells stained using anti-human CD105 (MEM-229) purified antibody (concentration in sample 3 µg/ml, red-filled, GAM APC) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 3 µg/ml, black-dashed, GAM APC) in flow cytometry analysis (surface staining).
Immunocytochemistry staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue). Primary antibody dilution: 5 ug/ml.
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