CD105 Rabbit Polyclonal Antibody

SKU: orb312175

Description

CD105 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Cell Markers, Endothelial Cells, Molecular Biology, Surface Molecules, Tumor Biomarkers, Vascular, cardiovascular Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD105 (601-658/658aa)
• Target: ENG
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

END; Endoglin; ENG; FLJ41744; HHT1; ORW; ORW1; Osler Rendu Weber syndrome 1; RP11 228B15.2; CD 105; CD105 antigen; EGLN_HUMAN; AI528660; AI662476; S-endoglin; SN6.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CD105 antibody (orb312175), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (orb312175) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (orb312175) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (orb312175) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

ENG

UniProt

P17813

References

SV Schröger, J Becker, S Kaya, R Rahimi-Nedjat, K Sagheb, S Blatt Boosting angiogenesis in ovo by biofunctionalizing collagen membranes with platelet-rich fibrin and hyaluronic acid: Implications for regenerative oral surgery? researchsquare.com, (2025)

PubMed: