Anti-Hu beta2-Microglobulin Purified

• Catalog Number: orb43660
• Category: Antibodies
• Description: Anti-Hu beta2-Microglobulin Purified
• Clonality: Monoclonal
• Clone Number: B2M-02
• Tested applications: ELISA, FC, ICC, IHC-P, WB
• Reactivity: Human, Porcine
• Isotype: Mouse IgG1
• Immunogen: Purified isolated human beta2-microglobulin.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: liver. Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred. Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: beta2-Microglobulin
• Entrez: 567
• UniProt ID: P61769
• RRID: AB_10998569
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: B2M
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: liver. Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred. Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human β2-microglobulin using mouse monoclonal antibody B2M-02 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. β2-microglobulin was detected around 12 kDa.