Anti-Hu beta2-Microglobulin Purified

• Catalog Number: orb43652
• Category: Antibodies
• Description: Anti-Hu beta2-Microglobulin Purified
• Clonality: Monoclonal
• Clone Number: B2M-01
• Tested applications: ELISA, FC, ICC, IHC-P, IP, RIA, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Purified human beta2-microglobulin
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli. Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: beta2-Microglobulin
• Entrez: 567
• UniProt ID: P61769
• RRID: AB_10993330
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: B2M
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli. Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
• Expiration Date: 12 months from date of receipt.

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line; Lane 2: EL4 mouse lymphoblastic lymphoma cell line; Lane 3: U937 human histiocytic lymphoma cell line.

Separation of human monocytes (red-filled) from human beta2-Microglobulin negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human beta2-Microglobulin (B2M-01) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human beta2-Microglobulin (B2M-01) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).

Western blotting analysis of human β2-microglobulin using mouse monoclonal antibody B2M-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. β2-microglobulin was detected around 12 kDa.