Anti-Histone H2A.X Antibody

• Catalog Number: orb393068
• Category: Antibodies
• Description: Rabbit polyclonal antibody to H2AFX.
• Target: H2AFX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone H2A.X. The exact sequence is proprietary.
• UniProt ID: P27661, P16104
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H2AX; Histone H2AX; H2a/x; Histone H2A.X
• Note: For research use only
• Entrez: 3014, 15270

Western blot analysis of Histone H2A.X expression in Panc1 (A), HCT116 (B), A549 (C), rat testis (D), CT26 (E) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunohistochemical analysis of Histone H2A.X staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H2A.X staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.