Anti-Histone H2A.X Antibody

• Catalog Number: orb334670
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H2A.X
• Target: H2AFX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of mouse Histone H2A.X. The exact sequence is proprietary.
• UniProt ID: P27661
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti H2AZ antibody, anti Histone H2A.Z antibody, a
• Note: For research use only
• Entrez: 15270

Western blot analysis of Histone H2A.X expression in NIH3T3 (A), rat brain (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Immunohistochemical analysis of Histone H2A.X staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.