HER2 Antibody

• Catalog Number: orb705572
• Category: Antibodies
• Description: Mouse monoclonal antibody to HER2
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human HER2. The exact sequence is proprietary.
• UniProt ID: P04626
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HER2; MLN19; NEU; NGL; Receptor tyrosine-protein k
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 2064
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HER2 expression in MDAMB231 (A), Hela (B) whole cell lysates. (Predicted band size: 137 kD; Observed band size: 180 kD)

Immunohistochemical analysis of HER2 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.