Anti-c-erbB-2 [FWP51]

• Catalog Number: orb669759
• Category: Antibodies
• Description: Mouse monoclonal antibody to c-erbB-2
• Target: c-erbB-2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: 20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.
• UniProt ID: P04626
• Tested applications: FC, IF, IP
• Clone Number: FWP51
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD340; Receptor tyrosine-protein kinase erbB-2; Ne
• Research Area: Cancer Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-c-erbB-2 antibody FWP51. Paraformaldehyde fixed MCF7 cells were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of FWP51 (orb669758, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells using anti-c-erbB-2 FWP51. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of FWP51 (orb669758) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669758, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.