C-erbB-2/HER2 Mouse Monoclonal Antibody

• Catalog Number: orb499961
• Category: Antibodies
• Description: C-erbB-2/HER2 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 13H9
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human HER2 protein full length
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 138 kDa
• Target: ERBB2
• UniProt ID: P04626
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Erb-B2 Receptor Tyrosine Kinase 2; NGL; NEU; V-Erb
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C-erbB-2, HER2) Monoclonal Antibody, Unconjugated (ascites of orb499961 13H9) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C-erbB-2/HER2) Monoclonal Antibody, conjugated (orb499961) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C-erbB-2/HER2) Monoclonal Antibody, Unconjugated (orb499961) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: SW480 (Human) Cell Lysate at 30 ug, Lane 3: Siha (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-C-erbB-2/HER2 (orb499961) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 185/100-110 kD, Observed band size: 185 kD.

Sample: Lane 1: Human A431 cell lysates, Lane 2: Human LOVO cell lysates, Lane 3: Human HeLa cell lysates, Primary: Anti-C-erbB-2/HER2 (orb499961) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 138 kDa, Observed band size: 180 kDa.