HER2 Mouse Monoclonal Antibody

SKU: orb323151

Description

The HER2 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human HER2. The exact sequence is proprietary. Purification: Affinity chromatography

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human HER2. The exact sequence is proprietary.
• Target: ERBB2
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340

Western blot analysis of HER2 expression in Hela (A), 293 (B), Jurkat (C) whole cell lysates. (Predicted band size: 137 kD; Observed band size: 185 kD)

Immunohistochemical analysis of HER2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

ERBB2

UniProt

P04626

NCBI Gene ID

2064