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Anti-HDAC6 Purified
Catalog Number: orb699853
| Catalog Number | orb699853 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonalantibody to HDAC6 |
| Clonality | Monoclonal |
| Clone Number | 178 |
| Tested applications | ELISA, ICC, IP |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | human HDAC6 |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | HDAC6 |
| Entrez | 10013 |
| UniProt ID | Q9NSW6 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-Histone deacetylase 6 antibody Read more... |
| Note | For research use only |
| Application notes | Immunocytochemistry: Recommended dilution: 5-10 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
ELISA analysis of human HDAC6 by mouse monoclonal antibody 178. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.
Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells by mouse monoclonal antibody 178, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue).
Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and 236 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.
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