HDAC6 Antibody

SKU: orb699853

Description

Mouse monoclonalantibody to HDAC6

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, ICC, IP
• Reactivity: Human
• Application Notes:
  Immunocytochemistry: Recommended dilution: 5-10 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 178
• Immunogen: human HDAC6
• Target: HDAC6
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Histone deacetylase 6

ELISA analysis of human HDAC6 by mouse monoclonal antibody 178. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.

Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells by mouse monoclonal antibody 178, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue).

Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and 236 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.

Quick Database Links

Gene Symbol

HDAC6

UniProt

Q9NSW6

NCBI Gene ID

10013