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Anti-HDAC6 Purified

Catalog Number: orb699854

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb699854
CategoryAntibodies
DescriptionMouse monoclonalantibody to HDAC6
ClonalityMonoclonal
Clone Number236
Tested applicationsELISA, FC, ICC, IP, WB
ReactivityHuman
IsotypeMouse IgG2b kappa
Immunogenhuman HDAC6
Antibody TypePrimary Antibody
Concentration1 mg/ml
PurityPurified by protein-A affinity chromatography.
ConjugationUnconjugated
TargetHDAC6
Entrez10013
UniProt IDQ9NSW6
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alternative namesAnti-Histone deacetylase 6 antibody
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NoteFor research use only
Application notesWestern blotting: Recommended dilution: 1-5 μg/ml.Immunocytochemistry: Recommended dilution: 5-10 μg/ml.Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining.
Expiration Date12 months from date of receipt.
Anti-HDAC6 Purified

ELISA analysis of human HDAC6 by mouse monoclonal antibody 236. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.

Anti-HDAC6 Purified

Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 μg/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Anti-HDAC6 Purified

Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 236, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).

Anti-HDAC6 Purified

Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and 236 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.

Anti-HDAC6 Purified

Western blotting analysis of human HDAC6 by mouse monoclonal antibody 236 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 µg/ml), followed by HRP-conjugated anti-mouse secondary antibody.

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