HDAC6 Antibody

• Catalog Number: orb699854
• Category: Antibodies
• Description: Mouse monoclonalantibody to HDAC6
• Target: HDAC6
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: human HDAC6
• UniProt ID: Q9NSW6
• Tested applications: ELISA, FC, ICC, IP, WB
• Application notes: Western blotting: Recommended dilution: 1-5 μg/ml.Immunocytochemistry: Recommended dilution: 5-10 μg/ml.Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: 236
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Histone deacetylase 6
• Note: For research use only
• Entrez: 10013
• Expiration Date: 12 months from date of receipt.

ELISA analysis of human HDAC6 by mouse monoclonal antibody 236. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.

Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 μg/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 236, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).

Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and 236 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.

Western blotting analysis of human HDAC6 by mouse monoclonal antibody 236 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 µg/ml), followed by HRP-conjugated anti-mouse secondary antibody.