Anti-HDAC6 Purified

• Catalog Number: orb699852
• Category: Antibodies
• Description: Mouse monoclonalantibody to HDAC6
• Clonality: Monoclonal
• Clone Number: 3D2
• Tested applications: ELISA, ICC, WB
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: human HDAC6
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HDAC6
• Entrez: 10013
• UniProt ID: Q9NSW6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Histone deacetylase 6 antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-5 μg/ml.Immunocytochemistry: Recommended dilution: 5-10 μg/ml.
• Expiration Date: 12 months from date of receipt.

ELISA analysis of human HDAC6 by mouse monoclonal antibody 3D2. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.

Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 3D2, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).

Western blotting analysis of human HDAC6 by mouse monoclonal antibody 3D2 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 µg/ml), followed by HRP-conjugated anti-mouse secondary antibody.