HDAC6 Antibody

SKU: orb699852

Description

Mouse monoclonalantibody to HDAC6

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Recommended dilution: 1-5 μg/ml.Immunocytochemistry: Recommended dilution: 5-10 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 3D2
• Immunogen: human HDAC6
• Target: HDAC6
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Histone deacetylase 6

ELISA analysis of human HDAC6 by mouse monoclonal antibody 3D2. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.

Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 3D2, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).

Western blotting analysis of human HDAC6 by mouse monoclonal antibody 3D2 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 µg/ml), followed by HRP-conjugated anti-mouse secondary antibody.

Quick Database Links

Gene Symbol

HDAC6

UniProt

Q9NSW6

NCBI Gene ID

10013