Anti-HDAC6 Purified

• Catalog Number: orb669265
• Category: Antibodies
• Description: Mouse monoclonal antibody to HDAC6
• Target: HDAC6
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified by protein-A affinity chromatography.
• Immunogen: human HDAC6
• UniProt ID: Q9NSW6
• Tested applications: ELISA, FC, ICC, WB
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: 159
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Histone deacetylase 6 antibody
• Note: For research use only
• Entrez: 10013

ELISA analysis of human HDAC6 by mouse monoclonal antibody 159. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.

Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4, 0 μg/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 159, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).