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Catalog Number | orb669265 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to HDAC6 |
Clonality | Monoclonal |
Clone Number | 159 |
Tested applications | ELISA, FC, ICC, WB |
Reactivity | Human |
Isotype | Mouse IgG1 kappa |
Immunogen | human HDAC6 |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | HDAC6 |
Entrez | 10013 |
UniProt ID | Q9NSW6 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-Histone deacetylase 6 antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
ELISA analysis of human HDAC6 by mouse monoclonal antibody 159. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.
Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4, 0 μg/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 159, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).