Anti-GPx-4 Antibody

• Catalog Number: orb340797
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPX4
• Target: GPX4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant full length protein of human GPx-4
• UniProt ID: P36969
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:10-1:100
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Phospholipid hydroperoxide glutathione peroxidase
• Note: For research use only
• Entrez: 2879

References

G Tian, J Li, W Wang, L Zhou FGF12 restrains mitochondria‐dependent ferroptosis in doxorubicin‐induced cardiomyocytes through the activation of FGFR1/AMPK/NRF2 signaling Drug Development Research, (2024)

PubMed:

Applications

WB

Reactivity

Mouse

Xiaoshan Chen 1, Wei Wang 2, Wei Wei TRIM22 mechanism promoting KAT2A ubiquitination degradation to regulate ferroptosis in hepatocellular carcinoma cell invasion and metastasis Histol Histopathol ., (2024)

PubMed: 39698827

F Ye, Y Xu, X Zhu, Q Ding, Y Wang, S Lu, Y Chen The mechanism of E3 ubiquitin ligase HERC1 regulating ferroptosis in lung adenocarcinoma cells Cancer Genetics, (2025)

PubMed:

Applications

WB

Reactivity

Human

Li, Meng et al. Melatonin Inhibits the Ferroptosis Pathway in Rat Bone Marrow Mesenchymal Stem Cells by Activating the PI3K/AKT/mTOR Signaling Axis to Attenuate Steroid-Induced Osteoporosis Oxid Med Cell Longev, 2022, 8223737 (2022)

PubMed: 36035224

Ma, Xiaojun et al. Lidocaine attenuates hypoxia/reoxygenation?induced inflammation, apoptosis and ferroptosis in lung epithelial cells by regulating the p38 MAPK pathway Mol Med Rep, 25, 150 (2022)

PubMed: 35244190

Western blot analysis of GPx-4 expression in mouse kidney (A) whole cell lysates. (Predicted band size: 19; 22 kD; Observed band size: 22 kD)

Immunohistochemical analysis of GPx-4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GPx-4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.