Glutathione Peroxidase 4/GPX4 Antibody

SKU: orb1098079

Description

Anti-Glutathione Peroxidase 4/GPX4 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
• Molecular Weight: 19 kDa
• Purification: Immunogen affinity purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Interferon alpha/beta receptor 2; IFN-R-2; IFN-alpha binding protein; IFN-alpha/beta receptor 2; Interferon alpha binding protein; Type I interferon receptor 2; IFNAR2; IFNABR; IFNARB

Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 19 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 19 kDa.

Quick Database Links

UniProt

P36969