GPX4 Rabbit Polyclonal Antibody

SKU: orb5346

This product can be custom made

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Description

GPX4 Rabbit Polyclonal Antibody

Research Area

Antioxidants, Cancer Research, Cell Biology, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Porcine, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4 (101-197/197aa)
Target	GPX4
Molecular Weight	20-22 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.

Success by Application

Reactivity Distribution

Latest Experiments

9 Results

Dilution

Sample

Summary

Mouse

Tumor tissue lysates

Tumor tissues were lysed to extract...

Human

1:1000

Human kidney proximal tubular (HK‑2) cell lysates

Cells were lysed in RIPA buffer,...

Human

1:100

Human renal tubular epithelial cells

HK‑2 cells were cultured on coverslips...

Chicken

Primary chicken hepatocytes

Cells were lysed in RIPA buffer...

Rat

1:100

Rat kidney sections

Rat kidneys were fixed in 4%...

1 2 Next

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx; mtPHGPx; GPX4_HUMAN; GPX4; Glutathione peroxidase 4 (GPx-4 | GSHPx-4); 1.11.1.12; GPX4_MOUSE; GPX4_RAT;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Sample: Kidney (mouse) Lysate at 40 ug, Primary: Anti-GPX4 (orb5346) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22kD, Observed band size: 20 kD.

Sample: Lane 1: Testis (Mouse) Tissue Lysate at 40 ug, Lane 2: Liver (Mouse) Tissue Lysate at 40 ug, Lane 3: Kidney (Mouse) Tissue Lysate at 40 ug, Lane 4: Heart (Mouse) Tissue Lysate at 40 ug, Lane 5: Testis (Rat) Tissue Lysate at 40 ug, Lane 6: Liver (Rat) Tissue Lysate at 40 ug, Lane 7: Kidney (Rat) Tissue Lysate at 40 ug, Lane 8: Heart (Rat) Tissue Lysate at 40 ug, Lane 9: U937 (Human) Cell Lysate at 30 ug, Lane 10: HepG2 (Human) Cell Lysate at 30 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: Huvec (Human) Cell Lysate at 30 ug, Lane 13: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-GPX4 (orb5346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 20-22 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-GPX4 (orb5346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 19 kD.

Quick Database Links

Gene Symbol

GPX4

UniProt

P36969

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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