GPX4 Rabbit Polyclonal Antibody

• Catalog Number: orb5346
• Category: Antibodies
• Description: GPX4 Rabbit Polyclonal Antibody
• Target: GPX4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4 (101-197/197aa)
• UniProt ID: P36969
• RRID: AB_10955955
• MW: 22 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4
• Note: For research use only

Sample: Kidney (mouse) Lysate at 40 ug, Primary: Anti-GPX4 (orb5346) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22kD, Observed band size: 20 kD.

Sample: Lane 1: Testis (Mouse) Tissue Lysate at 40 ug, Lane 2: Liver (Mouse) Tissue Lysate at 40 ug, Lane 3: Kidney (Mouse) Tissue Lysate at 40 ug, Lane 4: Heart (Mouse) Tissue Lysate at 40 ug, Lane 5: Testis (Rat) Tissue Lysate at 40 ug, Lane 6: Liver (Rat) Tissue Lysate at 40 ug, Lane 7: Kidney (Rat) Tissue Lysate at 40 ug, Lane 8: Heart (Rat) Tissue Lysate at 40 ug, Lane 9: U937 (Human) Cell Lysate at 30 ug, Lane 10: HepG2 (Human) Cell Lysate at 30 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: Huvec (Human) Cell Lysate at 30 ug, Lane 13: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-GPX4 (orb5346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 20-22 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-GPX4 (orb5346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 19 kD.