Anti-GLI2 Antibody

• Catalog Number: orb1743824
• Category: Antibodies
• Description: Anti-GLI2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Rat
• Immunogen: E.coli-derived human GLI2 recombinant protein (Position: A46-A1398).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 220 kDa
• UniProt ID: P10070
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-GLI2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of GLI2 using anti-GLI2 antibody.Lane 1:human 293T cell;2:human A549 cell;3:human U20S cell;4:rat brain tissue.

IF analysis of GLI2 using anti-GLI2 antibody and anti-Beta Tubulin antibody.GLI2 was detected in immunocytochemical section of U2OS cell.

IHC analysis of GLI2 using anti-GLI2 antibody. GLI2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue.

IHC analysis of GLI2 using anti-GLI2 antibody. GLI2 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue.

IHC analysis of GLI2 using anti-GLI2 antibody. GLI2 was detected in a paraffin-embedded section of human tonsil tissue.