GLI2 Rabbit Polyclonal Antibody

SKU: orb329628

Description

Rabbit polyclonal antibody to GLI2

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2
• Target: GLI2
• Molecular Weight: 133kDa
• Purification: Protein A purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

anti HPE9 antibody, anti THP1 antibody, anti THP2 antibody

Sample Tissue: Human RPMI 8226 Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Type: HepG2 Whole Cell lysates, Antibody Dilution: 1.0 ug/mL.

Sample Type: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5 ug/mL, Peptide Concentration: 4.0 ug/mL, Lysate Quantity: 25 ug/lane, Gel Concentration: 6%-18%.

Rabbit Anti-GLI2 Antibody, Paraffin Embedded Tissue: Human Heart, Cellular Data: Myocardial cells, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

Rabbit Anti-GLI2 Antibody, Paraffin Embedded Tissue: Human Intestine, Cellular Data: Epithelial cells of intestinal gland, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

Sample Type: Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop, Primary Antibody Dilution: 1:50, Secondary Antibody: Goat anti-rabbit-cy3, Secondary Antibody Dilution: 1:1500, Color/Signal Descriptions: GLI2: Green Neural crest cells: Red, Gene Name: GLI2.

Quick Database Links

Gene Symbol

GLI2

UniProt

P10070

RefSeq (Protein):NP_084655