GLI2 Rabbit Polyclonal Antibody

• Catalog Number: orb329627
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GLI2
• Target: GLI2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Human
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2
• Protein Sequence: Synthetic peptide located within the following region: RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE
• UniProt ID: P10070
• MW: 133kDa
• Tested applications: IHC, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HPE9 antibody, anti THP1 antibody, anti THP2
• Note: For research use only
• NCBI: NP_084655

Sample Tissue: Human RPMI 8226 Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Type: HepG2 Whole Cell lysates, Antibody Dilution: 1.0 ug/mL.

Sample Type: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5 ug/mL, Peptide Concentration: 4.0 ug/mL, Lysate Quantity: 25 ug/lane, Gel Concentration: 6%-18%.

Rabbit Anti-GLI2 Antibody, Paraffin Embedded Tissue: Human Heart, Cellular Data: Myocardial cells, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

Rabbit Anti-GLI2 Antibody, Paraffin Embedded Tissue: Human Intestine, Cellular Data: Epithelial cells of intestinal gland, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

Sample Type: Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop, Primary Antibody Dilution: 1:50, Secondary Antibody: Goat anti-rabbit-cy3, Secondary Antibody Dilution: 1:1500, Color/Signal Descriptions: GLI2: Green Neural crest cells: Red, Gene Name: GLI2.