Anti-GAPDH Antibody

• Catalog Number: orb1474084
• Category: Antibodies
• Description: Mouse monoclonal antibody to GAPDH
• Target: GAPDH
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant fusion protein of human GAPDH. The exact sequence is proprietary.
• UniProt ID: P16858, P04797, P04406
• Tested applications: FC, IH, WB
• Dilution range: WB (1/1000 - 1/4000), IH (1/50 - 1/200), FC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GA
• Note: For research use only
• Entrez: 100042025, 24383, 2597, 14433

Western blot analysis of GAPDH expression in Hela (A), Jurkat (B), A549 (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 35 kD)

Immunohistochemical analysis of GAPDH staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Hela cells using Anti-GAPDH Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.