SFRP5 Antibody (Center)

SKU: orb1937045

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P-Leica - 1:500
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB32418
• Immunogen: This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the Central region of human SFRP5. Antigen Region: 190-219 aa.
• Target: SFRP5
• Molecular Weight: 35563 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3

All lanes: Anti-SFRP5 Antibody (Center) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Human pancreas lysate. Lane 3: Hela whole cell lysate. Lane 4: Mouse eyeball lysate. Lane 5: Mouse pancreas lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-SFRP5 Antibody (Center) at 1:2000 dilution + human pancreas lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human colon tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

SFRP5 Antibody (Center) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the SFRP5 antibody detected the SFRP5 protein (arrow).

Quick Database Links

Gene Symbol

SFRP5

UniProt

Q5T4F7

RefSeq (Protein):NP_003006.2