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SFRP5 Antibody (Center)
Catalog Number: orb1937045
| Catalog Number | orb1937045 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the Central region of human SFRP5. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q5T4F7 |
| MW | 35563 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500 |
| Antibody Type | Primary Antibody |
| Clone Number | RB32418 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Secreted frizzled-related protein 5, sFRP-5, Frizz Read more... |
| Note | For research use only |
| NCBI | NP_003006.2 |
All lanes: Anti-SFRP5 Antibody (Center) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Human pancreas lysate. Lane 3: Hela whole cell lysate. Lane 4: Mouse eyeball lysate. Lane 5: Mouse pancreas lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-SFRP5 Antibody (Center) at 1:2000 dilution + human pancreas lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded human colon tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
SFRP5 Antibody (Center) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the SFRP5 antibody detected the SFRP5 protein (arrow).
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