SFRP5 Antibody (Center)

• Catalog Number: orb1937045
• Category: Antibodies
• Description: SFRP5 Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q5T4F7
• MW: 35563 Da
• Tested applications: IHC-P, WB
• Dilution range: IHC-P-Leica - 1:500, WB - 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: FRP1B, SARP3
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-SFRP5 Antibody (Center) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Human pancreas lysate. Lane 3: Hela whole cell lysate. Lane 4: Mouse eyeball lysate. Lane 5: Mouse pancreas lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-SFRP5 Antibody (Center) at 1:2000 dilution + human pancreas lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human colon tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

SFRP5 Antibody (Center) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the SFRP5 antibody detected the SFRP5 protein (arrow).